p-eif4ebp1 (s65) antibody Search Results


anti p eif4ebp1 s65  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc anti p eif4ebp1 s65
Anti P Eif4ebp1 S65, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p-eif4ebp1 (s65) antibody  (ABclonal Biotechnology)
90
ABclonal Biotechnology p-eif4ebp1 (s65) antibody
P Eif4ebp1 (S65) Antibody, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peif4ebp1 s65 eukaryotic translation initiation factor 4e binding  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc peif4ebp1 s65 eukaryotic translation initiation factor 4e binding
Peif4ebp1 S65 Eukaryotic Translation Initiation Factor 4e Binding, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti p eif4ebp1 s65 antibody  (Proteintech)
95
Proteintech rabbit anti p eif4ebp1 s65 antibody
Antibody sources and dilutions
Rabbit Anti P Eif4ebp1 S65 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p eif4ebp1 t37 46  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc p eif4ebp1 t37 46
Antibody sources and dilutions
P Eif4ebp1 T37 46, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abd antibodies gapdh  (Serotech Inc)
90
Serotech Inc abd antibodies gapdh
Antibody sources and dilutions
Abd Antibodies Gapdh, supplied by Serotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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biosciences antibodies sqstm1  (Becton Dickinson)
90
Becton Dickinson biosciences antibodies sqstm1
Antibody sources and dilutions
Biosciences Antibodies Sqstm1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies gabarapl2  (Proteintech)
90
Proteintech antibodies gabarapl2
Antibody sources and dilutions
Antibodies Gabarapl2, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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myc tag  (Proteintech)
96
Proteintech myc tag
CALCOCO1 is regulated by MTOR and autophagy. (A) Protein immunoblot analyses with antibodies as shown, in WT and atg5–/– MEFs, in glucose– or galactose-containing media. MEFs were treated with vehicle (Veh) or 100 nM MLN0128 (MLN) for ~17 h. The ratio <t>of</t> <t>CALCOCO1:ACTB</t> is shown. (B) Immunoblot analyses of HEK293 cells treated for 24 h with Veh, 100 nM MLN, and 100 µM chloroquine (CQ) as labeled. The ratio of CALCOCO1:ACTB is shown. (C) Immunoblot analyses of MB231 cells treated for 24 h with Veh, 100 nM MLN, and 50 nM bafilomycin A1 (Baf) as labeled. The ratio of CALCOCO1:ACTB is shown. (D) Immunoblot analyses of WT, sgATG7 KO, and sgATG3 KO Hep3B cells, treated for 24 h with Veh or 100 nM MLN0128. The ratio of CALCOCO1:ACTB is shown. (E) Images of MB231 cells. Top: Immunofluorescent images of CALCOCO1-MYCDDK using <t>anti-MYC</t> (9E10) antibody and DAPI . Bottom: Images of GFP-CALCOCO1. Single-color maximum projection images are shown on the left. Dual-color orthogonal views from Z-stack images are shown on the right. Scale bar: 10 µm. (F) Immunoblot analyses of cytoplasmic and nuclear fractions from HEK293, MB231, and MCF7 cells treated for 24 h with Veh or 100 nM MLN
Myc Tag, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gabarapl2  (Proteintech)
93
Proteintech gabarapl2
CALCOCO1 is regulated by MTOR and autophagy. (A) Protein immunoblot analyses with antibodies as shown, in WT and atg5–/– MEFs, in glucose– or galactose-containing media. MEFs were treated with vehicle (Veh) or 100 nM MLN0128 (MLN) for ~17 h. The ratio <t>of</t> <t>CALCOCO1:ACTB</t> is shown. (B) Immunoblot analyses of HEK293 cells treated for 24 h with Veh, 100 nM MLN, and 100 µM chloroquine (CQ) as labeled. The ratio of CALCOCO1:ACTB is shown. (C) Immunoblot analyses of MB231 cells treated for 24 h with Veh, 100 nM MLN, and 50 nM bafilomycin A1 (Baf) as labeled. The ratio of CALCOCO1:ACTB is shown. (D) Immunoblot analyses of WT, sgATG7 KO, and sgATG3 KO Hep3B cells, treated for 24 h with Veh or 100 nM MLN0128. The ratio of CALCOCO1:ACTB is shown. (E) Images of MB231 cells. Top: Immunofluorescent images of CALCOCO1-MYCDDK using <t>anti-MYC</t> (9E10) antibody and DAPI . Bottom: Images of GFP-CALCOCO1. Single-color maximum projection images are shown on the left. Dual-color orthogonal views from Z-stack images are shown on the right. Scale bar: 10 µm. (F) Immunoblot analyses of cytoplasmic and nuclear fractions from HEK293, MB231, and MCF7 cells treated for 24 h with Veh or 100 nM MLN
Gabarapl2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies for retreg1  (Proteintech)
94
Proteintech antibodies for retreg1
CALCOCO1 is regulated by MTOR and autophagy. (A) Protein immunoblot analyses with antibodies as shown, in WT and atg5–/– MEFs, in glucose– or galactose-containing media. MEFs were treated with vehicle (Veh) or 100 nM MLN0128 (MLN) for ~17 h. The ratio <t>of</t> <t>CALCOCO1:ACTB</t> is shown. (B) Immunoblot analyses of HEK293 cells treated for 24 h with Veh, 100 nM MLN, and 100 µM chloroquine (CQ) as labeled. The ratio of CALCOCO1:ACTB is shown. (C) Immunoblot analyses of MB231 cells treated for 24 h with Veh, 100 nM MLN, and 50 nM bafilomycin A1 (Baf) as labeled. The ratio of CALCOCO1:ACTB is shown. (D) Immunoblot analyses of WT, sgATG7 KO, and sgATG3 KO Hep3B cells, treated for 24 h with Veh or 100 nM MLN0128. The ratio of CALCOCO1:ACTB is shown. (E) Images of MB231 cells. Top: Immunofluorescent images of CALCOCO1-MYCDDK using <t>anti-MYC</t> (9E10) antibody and DAPI . Bottom: Images of GFP-CALCOCO1. Single-color maximum projection images are shown on the left. Dual-color orthogonal views from Z-stack images are shown on the right. Scale bar: 10 µm. (F) Immunoblot analyses of cytoplasmic and nuclear fractions from HEK293, MB231, and MCF7 cells treated for 24 h with Veh or 100 nM MLN
Antibodies For Retreg1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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map1lc3c  (Cell Signaling Technology Inc)
91
Cell Signaling Technology Inc map1lc3c
CALCOCO1 interacts with <t>MAP1LC3C</t> and other LC3/GABARAP family members. (A) Cartoon of domains and motifs found in human and mouse CALCOCO family members. (B) Immunoprecipitation (IP) analyses of CALCOCO1-MYCDDK and HA-tagged MAP1LC3/GABARAP orthologs in HEK293 cells. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody, resolved by SDS-PAGE, and probed with anti-HA or anti-DDK antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as described. (C) IP analyses of CALCOCO1-MYCDDK and HA-tagged MAP1LC3/GABARAP orthologs expressed in HEK293 cells that were treated overnight with chloroquine (CQ) to inhibit lysosomal flux. HA-tagged protein complexes were IP’d with anti-HA antibody and probed with anti-DDK or mixed antibodies against HA, MAP1LC3A/B, and MAP1LC3C. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as described. (D) IP analyses of HA-MAP1LC3C or HA-GFP co-expressed with wild type (WT) CALCOCO1-MYCDDK, CLIR mutants L140A and V142A, and LIR mutant W47A in HEK293 cells. CALCOCO1W47A was transfected at 2 different concentrations to match WT expression levels. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody, and probed with anti-HA or anti-CALCOCO1 antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as noted. (E) IP analyses of CALCOCO1-MYCDDK with endogenous MAP1LC3B. WT CALCOCO1-MYCDDK, 2 concentrations of the CALCOCO1W47A, and GFP-DDK (control) were expressed in HEK293 cells, then treated for 18 h with 100 nM MLN0128 and 100 uM CQ. CALCOCO1-MYCDDK complexes were immunoprecipitated with anti-Flag® M2 affinity gel (MilliporeSigma, A2220) and probed with anti-DDK or MAP1LC3B antibodies. Expressions of CALCOCO1-MYCDDK, GFP-DDK, ACTB and MAP1LC3B in cell lysates are shown with antibodies as noted. (F) IP analyses of HA-MAP1LC3C or HA-GFP co-expressed with WT CALCOCO1-MYCDDK, CLIR mutants (L140A and V142A), and R12H mutant in HEK293 cells. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody and probed with anti-HA or anti-CALCOCO1 antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies, as noted
Map1lc3c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/map1lc3c/product/Cell Signaling Technology Inc
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Image Search Results


Antibody sources and dilutions

Journal: Journal of Translational Medicine

Article Title: TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury

doi: 10.1186/s12967-023-04027-4

Figure Lengend Snippet: Antibody sources and dilutions

Article Snippet: Rabbit-anti-P-EIF4EBP1-S65 antibody , Proteintech , #12721 , 1:2000.

Techniques: Western Blot, Immunofluorescence, Flow Cytometry, Staining

mTOR regulates TREM-1-induced mitochondrial fission in macrophages. A – C Protein levels of p-mTOR ser2448 and p-4E-BP1 S65 , n = 3. D - E , mitochondrial morphology was stained with TOM20 (green) and the morphological skeleton. F , quantification of mitochondrial morphology, n = 3. G , H Mitochondrial fission-related proteins: TOM20, DRP1 Ser616 , MTFP1, and PGAM5 proteins, n = 3. G , H Mitophagy-related proteins: Pink1 and Beclin1, n = 3

Journal: Journal of Translational Medicine

Article Title: TREM-1 triggers necroptosis of macrophages through mTOR-dependent mitochondrial fission during acute lung injury

doi: 10.1186/s12967-023-04027-4

Figure Lengend Snippet: mTOR regulates TREM-1-induced mitochondrial fission in macrophages. A – C Protein levels of p-mTOR ser2448 and p-4E-BP1 S65 , n = 3. D - E , mitochondrial morphology was stained with TOM20 (green) and the morphological skeleton. F , quantification of mitochondrial morphology, n = 3. G , H Mitochondrial fission-related proteins: TOM20, DRP1 Ser616 , MTFP1, and PGAM5 proteins, n = 3. G , H Mitophagy-related proteins: Pink1 and Beclin1, n = 3

Article Snippet: Rabbit-anti-P-EIF4EBP1-S65 antibody , Proteintech , #12721 , 1:2000.

Techniques: Staining

CALCOCO1 is regulated by MTOR and autophagy. (A) Protein immunoblot analyses with antibodies as shown, in WT and atg5–/– MEFs, in glucose– or galactose-containing media. MEFs were treated with vehicle (Veh) or 100 nM MLN0128 (MLN) for ~17 h. The ratio of CALCOCO1:ACTB is shown. (B) Immunoblot analyses of HEK293 cells treated for 24 h with Veh, 100 nM MLN, and 100 µM chloroquine (CQ) as labeled. The ratio of CALCOCO1:ACTB is shown. (C) Immunoblot analyses of MB231 cells treated for 24 h with Veh, 100 nM MLN, and 50 nM bafilomycin A1 (Baf) as labeled. The ratio of CALCOCO1:ACTB is shown. (D) Immunoblot analyses of WT, sgATG7 KO, and sgATG3 KO Hep3B cells, treated for 24 h with Veh or 100 nM MLN0128. The ratio of CALCOCO1:ACTB is shown. (E) Images of MB231 cells. Top: Immunofluorescent images of CALCOCO1-MYCDDK using anti-MYC (9E10) antibody and DAPI . Bottom: Images of GFP-CALCOCO1. Single-color maximum projection images are shown on the left. Dual-color orthogonal views from Z-stack images are shown on the right. Scale bar: 10 µm. (F) Immunoblot analyses of cytoplasmic and nuclear fractions from HEK293, MB231, and MCF7 cells treated for 24 h with Veh or 100 nM MLN

Journal: Autophagy

Article Title: Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy

doi: 10.1080/15548627.2020.1719746

Figure Lengend Snippet: CALCOCO1 is regulated by MTOR and autophagy. (A) Protein immunoblot analyses with antibodies as shown, in WT and atg5–/– MEFs, in glucose– or galactose-containing media. MEFs were treated with vehicle (Veh) or 100 nM MLN0128 (MLN) for ~17 h. The ratio of CALCOCO1:ACTB is shown. (B) Immunoblot analyses of HEK293 cells treated for 24 h with Veh, 100 nM MLN, and 100 µM chloroquine (CQ) as labeled. The ratio of CALCOCO1:ACTB is shown. (C) Immunoblot analyses of MB231 cells treated for 24 h with Veh, 100 nM MLN, and 50 nM bafilomycin A1 (Baf) as labeled. The ratio of CALCOCO1:ACTB is shown. (D) Immunoblot analyses of WT, sgATG7 KO, and sgATG3 KO Hep3B cells, treated for 24 h with Veh or 100 nM MLN0128. The ratio of CALCOCO1:ACTB is shown. (E) Images of MB231 cells. Top: Immunofluorescent images of CALCOCO1-MYCDDK using anti-MYC (9E10) antibody and DAPI . Bottom: Images of GFP-CALCOCO1. Single-color maximum projection images are shown on the left. Dual-color orthogonal views from Z-stack images are shown on the right. Scale bar: 10 µm. (F) Immunoblot analyses of cytoplasmic and nuclear fractions from HEK293, MB231, and MCF7 cells treated for 24 h with Veh or 100 nM MLN

Article Snippet: Antibodies Primary antibodies used in this study include: Abcam antibodies for CALCOCO1/CoCoA (ab70564); ABD SeroTech antibodies GAPDH (HCA272); BD Biosciences antibodies for SQSTM1 (610832) and RPS6KB1 (611261); Cell Signaling Technology antibodies for ATG3 (3415), ATG5 (8540), ATG7 (8558), MAP1LC3A/B (12741), MAP1LC3C (14723), ULK1 (6439 and 8054), p-EIF4EBP1 T37/46 (2855), p-EIF4EBP1 S65 (9456), EIF4EBP1 (9644) p-AKT1 S473 (4060), AKT1 (9272) p-RPS6KB T389 (9234), p-ACACA S79 (11818), ACACA (3662) DYKDDDDK-tag (14793), MYC-tag (2276), ACTB (beta-Actin; 3700), LMNA (lamin A/C; 4777); Novus antibodies for MAP1LC3B (NB100-2220); Protein Tech antibodies for RETREG1 (21537-1-AP), GABARAPL2 (18724-1-AP), MAP1LC3C (18726-1-AP); Santa Cruz Biotechnology antibodies for CALCOCO1 (sc-515670), HA-tag (sc-805), GST-tag (sc-138), TUBA (alpha-tubulin; sc-5286); MilliporeSigma antibody for Flag M2 (F1804).

Techniques: Western Blot, Labeling

CALCOCO1 interacts with MAP1LC3C and other LC3/GABARAP family members. (A) Cartoon of domains and motifs found in human and mouse CALCOCO family members. (B) Immunoprecipitation (IP) analyses of CALCOCO1-MYCDDK and HA-tagged MAP1LC3/GABARAP orthologs in HEK293 cells. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody, resolved by SDS-PAGE, and probed with anti-HA or anti-DDK antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as described. (C) IP analyses of CALCOCO1-MYCDDK and HA-tagged MAP1LC3/GABARAP orthologs expressed in HEK293 cells that were treated overnight with chloroquine (CQ) to inhibit lysosomal flux. HA-tagged protein complexes were IP’d with anti-HA antibody and probed with anti-DDK or mixed antibodies against HA, MAP1LC3A/B, and MAP1LC3C. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as described. (D) IP analyses of HA-MAP1LC3C or HA-GFP co-expressed with wild type (WT) CALCOCO1-MYCDDK, CLIR mutants L140A and V142A, and LIR mutant W47A in HEK293 cells. CALCOCO1W47A was transfected at 2 different concentrations to match WT expression levels. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody, and probed with anti-HA or anti-CALCOCO1 antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as noted. (E) IP analyses of CALCOCO1-MYCDDK with endogenous MAP1LC3B. WT CALCOCO1-MYCDDK, 2 concentrations of the CALCOCO1W47A, and GFP-DDK (control) were expressed in HEK293 cells, then treated for 18 h with 100 nM MLN0128 and 100 uM CQ. CALCOCO1-MYCDDK complexes were immunoprecipitated with anti-Flag® M2 affinity gel (MilliporeSigma, A2220) and probed with anti-DDK or MAP1LC3B antibodies. Expressions of CALCOCO1-MYCDDK, GFP-DDK, ACTB and MAP1LC3B in cell lysates are shown with antibodies as noted. (F) IP analyses of HA-MAP1LC3C or HA-GFP co-expressed with WT CALCOCO1-MYCDDK, CLIR mutants (L140A and V142A), and R12H mutant in HEK293 cells. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody and probed with anti-HA or anti-CALCOCO1 antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies, as noted

Journal: Autophagy

Article Title: Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy

doi: 10.1080/15548627.2020.1719746

Figure Lengend Snippet: CALCOCO1 interacts with MAP1LC3C and other LC3/GABARAP family members. (A) Cartoon of domains and motifs found in human and mouse CALCOCO family members. (B) Immunoprecipitation (IP) analyses of CALCOCO1-MYCDDK and HA-tagged MAP1LC3/GABARAP orthologs in HEK293 cells. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody, resolved by SDS-PAGE, and probed with anti-HA or anti-DDK antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as described. (C) IP analyses of CALCOCO1-MYCDDK and HA-tagged MAP1LC3/GABARAP orthologs expressed in HEK293 cells that were treated overnight with chloroquine (CQ) to inhibit lysosomal flux. HA-tagged protein complexes were IP’d with anti-HA antibody and probed with anti-DDK or mixed antibodies against HA, MAP1LC3A/B, and MAP1LC3C. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as described. (D) IP analyses of HA-MAP1LC3C or HA-GFP co-expressed with wild type (WT) CALCOCO1-MYCDDK, CLIR mutants L140A and V142A, and LIR mutant W47A in HEK293 cells. CALCOCO1W47A was transfected at 2 different concentrations to match WT expression levels. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody, and probed with anti-HA or anti-CALCOCO1 antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies as noted. (E) IP analyses of CALCOCO1-MYCDDK with endogenous MAP1LC3B. WT CALCOCO1-MYCDDK, 2 concentrations of the CALCOCO1W47A, and GFP-DDK (control) were expressed in HEK293 cells, then treated for 18 h with 100 nM MLN0128 and 100 uM CQ. CALCOCO1-MYCDDK complexes were immunoprecipitated with anti-Flag® M2 affinity gel (MilliporeSigma, A2220) and probed with anti-DDK or MAP1LC3B antibodies. Expressions of CALCOCO1-MYCDDK, GFP-DDK, ACTB and MAP1LC3B in cell lysates are shown with antibodies as noted. (F) IP analyses of HA-MAP1LC3C or HA-GFP co-expressed with WT CALCOCO1-MYCDDK, CLIR mutants (L140A and V142A), and R12H mutant in HEK293 cells. HA-tagged protein complexes were immunoprecipitated with anti-HA antibody and probed with anti-HA or anti-CALCOCO1 antibody. Expressions of tagged proteins and ACTB in cell lysates are shown with antibodies, as noted

Article Snippet: Antibodies Primary antibodies used in this study include: Abcam antibodies for CALCOCO1/CoCoA (ab70564); ABD SeroTech antibodies GAPDH (HCA272); BD Biosciences antibodies for SQSTM1 (610832) and RPS6KB1 (611261); Cell Signaling Technology antibodies for ATG3 (3415), ATG5 (8540), ATG7 (8558), MAP1LC3A/B (12741), MAP1LC3C (14723), ULK1 (6439 and 8054), p-EIF4EBP1 T37/46 (2855), p-EIF4EBP1 S65 (9456), EIF4EBP1 (9644) p-AKT1 S473 (4060), AKT1 (9272) p-RPS6KB T389 (9234), p-ACACA S79 (11818), ACACA (3662) DYKDDDDK-tag (14793), MYC-tag (2276), ACTB (beta-Actin; 3700), LMNA (lamin A/C; 4777); Novus antibodies for MAP1LC3B (NB100-2220); Protein Tech antibodies for RETREG1 (21537-1-AP), GABARAPL2 (18724-1-AP), MAP1LC3C (18726-1-AP); Santa Cruz Biotechnology antibodies for CALCOCO1 (sc-515670), HA-tag (sc-805), GST-tag (sc-138), TUBA (alpha-tubulin; sc-5286); MilliporeSigma antibody for Flag M2 (F1804).

Techniques: Immunoprecipitation, SDS Page, Mutagenesis, Transfection, Expressing